http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103290034-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6c6d22af4bf4e1b2205a929549da46d3 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 |
| filingDate | 2012-03-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b137b74634eaf632368702ea54500017 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5ef39f30ef23cd96a408127cc0efbf48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_06c970765bb8b2c97e9be0fc5f691b7a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6443f02da40014687789b9f9817753b8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_466bc4e896667e60e63ce918185df381 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_44f2d621637ef808e5525bd3501d91c8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4fe972f42739e52bb95ba84e7e26e88b |
| publicationDate | 2015-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103290034-B |
| titleOfInvention | Ubiquitin ligase gene participating in symbiotic nodulation of leguminous plants, and application thereof |
| abstract | The invention discloses a ubiquitin ligase gene participating in symbiotic nodulation of leguminous plants and application thereof. A membrane outer part of crowtoe symbiotic receptor kinase serves as bait; partial sequence of the gene is screened from a crowtoe AD-cDNA library; and full-length sequence is obtained through amplification by gene splicing and reverse transcription-polymerase chain reaction (RT-PCR) methods according to two expression sequence tags Lj-G0027263 and Lj-G0032470 overlapped with the partial sequence. Gene cloning and sequencing comparative analysis proves that the gene is cloned from crowtoe genome and cDNA. Sequence analysis and in vitro ubiquitin activity test prove that the gene is novel ubiquitin ligase. According to the application of the gene to the leguminous plants, the biological function of the gene in a symbiotic nodulation process is researched by RNAi and overexpression methods. The results show that, compared with the control plants, the plants with he gene has the advantages that the number of root nodules is increased obviously after overexpression, and the number of root nodules is reduced obviously after RNAi, so the gene has an application prospect in symbiotic nitrogen fixation of the leguminous plants. |
| priorityDate | 2012-03-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 118.