http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103289979-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_20cdf16355412916f9ddcf9598336cf7 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-07 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N13-00 |
| filingDate | 2012-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_459285e865dc39574064819626b340d4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d91872b66f36fd931124b1ab1217ae2b |
| publicationDate | 2013-09-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103289979-A |
| titleOfInvention | Method for preparing alkaline protease by fermentation method |
| abstract | The invention provides a method for preparing an alkaline protease by a fermentation method. The method comprises the following steps of: obtaining a high-yield bacillus alcalophilus strain Svf4-21-7 stable in heritability through ultraviolet-NET compound mutation treatment; carrying out the following treatment steps on the fermentation broth: salting out by using ammonium sulfate, dialysis and desalination, ion-exchange column chromatography, polyethylene glycol embedment and enrichment and gel filtering chromatography, thus obtaining the alkaline protease having activity greater than or equal to 100000 u/g after being freeze-dried. The alkaline protease prepared by the method provided by the invention is high in yield, and the preparation process is relatively simple; therefore, the alkaline protease is suitable for industrial production. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110408568-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103509784-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106082767-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-106082767-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103333873-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110408568-B |
| priorityDate | 2012-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 52.