http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103278627-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 |
filingDate | 2013-05-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2015-09-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2015-09-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103278627-B |
titleOfInvention | A kind of multi-antigen ELISA kit detecting African swine fever virus antibody |
abstract | One of the present invention detects many antigen enzyme linked immunosorbent assay (ELISA) kit of African swine fever virus (ASFV) antibody and belongs to biotechnology and zoonosis diagnosis research field, comprises three kinds of ASFV recombinant antigen expression and purification, ASFV antibody positive and negative control seras preparations, the combination of best envelope antigen and concentration is determined, the optimization of multi-antigen ELISA (MA-ELISA) response parameter, ASFV negative antibody serum critical value are determined and MA-ELISA detects the sensitivity of artificial challenge and field blood serum sample, specificity, repeatablely to measure.Detected by a large amount of known blood serum sample and prove, MA-ELISA of the present invention detects the sensitivity of serum ASFV antibody, specificity and repeatability and is significantly higher than the ELISA method and external similar kit that OIE recommends, and can be used for ASFV serodiagnosis, epidemiology survey and live pig and imports and exports quarantine and examination. |
priorityDate | 2013-05-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 27.