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filingDate 2013-06-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2014-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_410b50fc7e5d220916ffdbc6f043b903
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publicationDate 2014-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-103275999-B
titleOfInvention Apolygus lucorum membrane-bound trehalase, its coding sequence, vector and strain of sequence, and application of vector or strain
abstract The invention discloses an Apolygus lucorum membrane-bound trehalase coding DNA sequence. The invention also discloses an Apolygus lucorum membrane-bound trehalase, a recombinant expression vector or a transgenic cell system or a transgenic transgenic bacterium of the Apolygus lucorum membrane-bound trehalase coding DNA sequence, an application of the recombinant expression vector or the transgenic cell system or the transgenic transgenic bacterium in the production of the Apolygus lucorum membrane-bound trehalase, and a preparation method of the Apolygus lucorum membrane-bound trehalase. The molecular weight of zymoprotein prepared in the invention is 60KD, and zymoprotein can degrade trehalose to form glucose at an optimal temperature of 50DEG C under an optimal pH value of 7.0. Purified trehalase can be used for the qualitative and quantitative content detection of trehalase in industries and other fields, and provides a foundation for the research and development of insect trehalase inhibitors.
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