http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103275272-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ea2c0d0dcb67d4a3c3831d91df0d6eb0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_464cc68b22156c1fa8704fb6364ea865 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08F226-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J20-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J20-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08F2-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-545 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08J9-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08J9-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08F222-14 |
| filingDate | 2013-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_85e89c7b2249a548c69b11a631cb2778 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0823662c7ed8a8f796df520112c58d6a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c987692ea045163a3ea64cf8d39d4b6d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_07a8ec6c4062802eade0e639bb053a35 |
| publicationDate | 2015-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103275272-B |
| titleOfInvention | Preparation method of 96-well enzyme label plate AOZ molecularly imprinted polymer film |
| abstract | The invention discloses a preparation method of a 96-well enzyme label plate AOZ molecularly imprinted polymer film. The preparation method comprises the following steps of a, dissolving AOZ as a template molecule, 2-vinylpyddine as a functional monomer and ethylene glycol dimethacrylate as a cross-linking agent in ethanol as a pore forming agent according to a mole ratio of 1: 4: 1, and carrying out ultrasonic treatment for 30min, wherein a use amount ratio of ethanol to AOZ is 5ml/0.25mmol, b, adding azodiisobutyronitrile as an initiator into the mixture, and carrying out ultrasonic treatment for 15min, wherein a use amount ratio of azodiisobutyronitrile to AOZ is 25mg/0.25mmol, c, dropwisely adding the solution obtained by the step b on a 96-well enzyme label plate, putting the 96-well enzyme label plate into a sealing bag, filling nitrogen into the sealing bag, carrying out sealing, and carrying out ultraviolet lamp irradiation to initiate polymerization lasting for 24h, d, putting the 96-well enzyme label plate into a methanol-acetic acid mixed solution having a volume ratio of 3: 1, and carrying out ultrasonic elution for 48h, and e, drying the 96-well enzyme label plate for 2h at a temperature of 50 DEG C to obtain the AOZ molecularly imprinted polymer film. |
| priorityDate | 2013-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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