http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103266175-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d6068f2f9b7c230583fb05b301f10881 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08F222-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08F220-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/D01D5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/D01F6-40 |
| filingDate | 2013-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_faaa8958adbad331c3f7e281d6a68c94 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d952d7d7203d1aeb9553d86cc350b10a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_10e601fb1528f5d9577084efc9fb15d3 |
| publicationDate | 2014-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103266175-B |
| titleOfInvention | Preparation method and application of thrombin aptamer functionalized electrospining enhanced solid-phase microextraction rod |
| abstract | The invention discloses preparation and application of a thrombin aptamer functionalized electrospining enhanced solid-phase microextraction rod. The thrombin aptamer functionalized electrospining enhanced solid-phase microextraction rod is prepared through the following steps of: synthesizing a polyacrylonitrile-maleic acid polymer by adopting a free radical water-phase precipitation polymerization method; then preparing a solid-phase extraction rod which has the advantages of large surface area, high stability and high durability by directly taking a stainless steel wire as a substrate and adopting an electrospining technology; and fixing a thrombin aptamer to the surface of the solid-phase extraction rod by adopting a carbodiimide method. The prepared thrombin aptamer functionalized electrospining enhanced solid-phase microextraction rod can be applied to the isolation analysis of a trace amount of thrombin contained in complex samples, such as human serum, human plasma, human blood, and the like. The method disclosed by the invention can be used for preparing a solid-phase microextraction base body coating and controlling the thickness of the solid-phase microextraction base body coating by taking the stainless steel wire as the substrate and adopting an electrospining technology; the solid-phase microextraction base body coating is uniform, loose, porous and large in surface area; and the prepared solid-phase microextraction rod has the advantages of high specificity and high stability. |
| priorityDate | 2013-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 40.