http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103266123-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7d13b253bd8988165c65d816362f7316 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_62fd70cba2aa0f3d0671e6f392868dd6 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65 |
| filingDate | 2013-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8e96193cca26a0adc68a3bc86dbd1627 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ca3265620b12b0311b924c15e5f57fef http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cba9bf157e01b2388793a5d5655e79d4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_348935cd6f9226f01f370f8c7f170182 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3475bd720c6a90200c785d0d23c13dd9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c0064e1a297419db7017e39da11f7ccf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9c9fc69aab3b5be4eafeced43e32f9f2 |
| publicationDate | 2014-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103266123-B |
| titleOfInvention | Construction method for expression vector of functional genes in large laboratory animal |
| abstract | A prokaryotic expression vector pET22b is applied as a basic vector in the invention for the implementation of design, vector construction and function verification. An expression vector of functional genes in a large laboratory animal with a high expression rate and a high transmembrane rate is obtained finally. The vector belongs to the field of cell and molecular biology. The expression vector of functional genes as constructed in the invention has a large quantity of enzyme digestion sites and a big scope for gene selection; the in-vitro transcriptional level of target genes can be regulated and controlled accurately. Expressed proteins can be introduced into mammalian cells. The transduction has a high efficiency and a high speed. The method is applicable for a research on gene functions. The expression vector of functional genes in a large laboratory animal related to in the invention can provide a convenient and effective gene delivery tool for genetic engineering, cell engineering, immunology and molecular biology as well as other life science researches; the expression vector can also proved a new way for the research on the genetic regulatory mechanism of large laboratory animals. |
| priorityDate | 2013-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 102.