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filingDate 2011-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2017-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2017-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-103261397-B
titleOfInvention It is ethanol that xylose isomerase and xylitol dehydrogenase, which are combined for wood-sugar fermentation, and bacteroides fragilis xylose dehydrogenase
abstract Disclosed here is a kind of newfound problem andsolution for following, is that xylulose is used to enter pentose phosphate pathway via Xylulokinase using xylose isomerase enzymatic conversion xylose, saccharomyces cerevisiae is engineered and carrys out xylose-fermenting manufacture ethanol.When being grown on a kind of culture medium for include xylose, xylitol is intended to accumulate in cell, although lacking xylose reductase activities in saccharomyces cerevisiae.Xylitol suppresses xylose isomerase activity.A kind of solution of description is to express a kind of external source xylitol dehydrogenase simultaneously together with the external source xylose isomerase while be also optionally overexpressed xylose kinase in the case where lacking a kind of Xylose reductase expression.Another solution is a kind of xylose isomerase from bacteroides fragilis, with other xylose isomerases(Such as Escherichia coli xylose isomerase)Compared to less being suppressed by xylitol.The expression of the bacteroides fragilis xylose isomerase can be used alone, or with the expression of xylitol dehydrogenase and is optionally applied in combination with the overexpression of Xylulokinase to improve the alcohol production from xylose.
priorityDate 2010-12-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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