http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103255140-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6c6d22af4bf4e1b2205a929549da46d3 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-84 |
| filingDate | 2012-02-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_960aa8102df741c1aee55d5b5ad4202d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6354a4ce3881e16787fb897f30f8b8f6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_323a1c345043af2f8cc8f8d2eedd5f4d |
| publicationDate | 2014-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103255140-B |
| titleOfInvention | Nitrogen supply recovery specific induced expression promoter Y2 after paddy rice nitrogen deficiency, and application thereof |
| abstract | The invention belongs to the technical field of plant genetic engineering, and specifically relates to a nitrogen supply recovery specific induced expression promoter Y2 after paddy rice nitrogen deficiency, and an application thereof. With a chip technology, promoter Y2 downstream gene nitrogen supply induced expression information is obtained. With different paddy rice varieties, Y2 downstream gene nitrogen supply induced expression mode is verified. With a PCR method, the promoter Y2 full length and a 5'-terminal truncated fragment thereof are amplified, and are connected to a promoter vector DX2181b, such that a promoter fusion GUS expression vector is obtained. The constructed vector is used in genetic transformation upon a paddy rice variety Zhonghua11. The regulation of the promoter upon GUS gene is verified in a transformation positive plant. Through Realtime expression level verification and GUS protein activity detection, it is further determined that the promoter is a nitrogen supply recovery specific induced expression promoter after nitrogen deficiency. A functional section is at AT6 upstream 519bp. |
| priorityDate | 2012-02-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 88.