http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103243155-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2013-01-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103243155-B |
titleOfInvention | The method for quick in cattle CIDEC gene mononucleotide polymorphism site and application |
abstract | The invention discloses a kind of method detecting the multiple single nucleotide polymorphism in cattle CIDEC gene 5 ' control region, with comprise CIDEC gene cattle complete genome DNA to be measured as template, with primer to P(P1, P2, P3) as primer, PCR expands cattle CIDEC geneļ¼Use restricted enzyme HaeIII, AccI, after HaeIII digests pcr amplification product respectively, again the amplified fragments after enzyme action is carried out agarose gel electrophoresis, cattle CIDEC gene the 974th is identified according to agarose gel electrophoresis result, 956th, the single nucleotide polymorphism of the 501st, because complete linkage, the polymorphism of the 956th also represent the 841st, 763, the polymorphism of 727 and 546, the polymorphism of the 501st also represent the single nucleotide polymorphism of the 643rd, so polymorphism in three sites of detection can be obtained by the polymorphism information in nine sites.The single nucleotide polymorphism that detection method is CIDEC gene of present invention offer is laid a good foundation with the foundation of growth traits relation, in order to for the marker assisted selection (MAS) of the meat growth traits of Chinese Cattle, quickly set up the cattle population that genetic resources is excellent. |
priorityDate | 2013-01-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 103.