http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103243076-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9aa1efd0345eed46ff33ee5af2b519b7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c3c5de33a1a2b6a75d0bd56b8137a6b4 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
| filingDate | 2012-02-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b9d9299eb821535e85dad78c76ceb24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_277adbec617b7990ec84bbdcce0d6820 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8779eaaa9364b20227c39cc0036ad74c |
| publicationDate | 2014-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103243076-B |
| titleOfInvention | Treatment method for preparing sperm of transgenic mouse |
| abstract | The invention belongs to a transgenic field, and relates to a treatment method for preparing sperm of a transgenic mouse. The treatment method for preparing sperm of the transgenic mouse, comprises: incubating mouse epididymal sperm at 37 DEG C in a 5% CO2 incubator for 8-10min, mixing a linear DNA fragment containing a target gene into the sperm, ice-bathing the sperm at 2-5 DEG C for 25-30min, heat-shocking the sperm at 40-45 DEG C for 1-3min, ice-bathing the sperm at 2-5 DEG C for 3-5min, and then incubating the sperm at 37 DEG C in a 5% CO2 incubator for 50-70min for capacitation The method can raise permeability of a sperm membrane, is convenient for introducing a gene fragment into the sperm, and raises transgenic efficiency. The invention solves the problems of low efficiency, bad stability and difficult passage when sperm is used to prepare the transgenic mouse. An effective method for preparing the transgenic mouse is disclosed. |
| priorityDate | 2012-02-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 33.