http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103233080-B
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_abd156263f0d78fc96518fafc94f377f |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 |
filingDate | 2013-05-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2014-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3b3d85364cf22319fd1919c56c1236f9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f340693b77c232f32e35471f7144e653 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a75af6291403cbb53bb677cc3c1bcebb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ace71305328a29380354a2b268558853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0b820267f09f92f54e87006ed4fdc1bf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b64eef46c149779bd233ab5bc587c31e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c6d32a9b91f7f428f0142dc8f45cd21a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8694488abdfadec40fcabda894ef5b5c |
publicationDate | 2014-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103233080-B |
titleOfInvention | Method for identifying mating types of Lepista sordida protoplast monokaryons and special primer pair SR-1*3 therefor |
abstract | The invention discloses a method for identifying the mating types of Lepista sordida protoplast monokaryons and a special primer pair SR-1*3 therefor. The method comprises the following steps: respectively taking genome DNAs (deoxyribonucleic acid) of two Lepista sordida protoplast monokaryons to be identified as templates, performing PCR (polymerase chain reaction) amplification by using a PCR primer pair composed of two single-chain DNAs as shown in SEQ ID NO.1 and SEQ ID NO.2, and detecting the sizes of the obtained PCR products; if both or neither of the PCR products of the two Lepista sordida protoplast monokaryons to be identified contains a DNA segment of 500-800bp, indicating that the mating types of the two Lepista sordida protoplast monokaryons to be identified are the same; and if one of the two contains a DNA segment of 500-800bp and the other does not contain a DNA segment of 500-800bp, indicating that the mating types of the two Lepista sordida protoplast monokaryons to be identified are different. |
priorityDate | 2013-05-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 35.