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grantDate 2014-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2014-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-103233080-B
titleOfInvention Method for identifying mating types of Lepista sordida protoplast monokaryons and special primer pair SR-1*3 therefor
abstract The invention discloses a method for identifying the mating types of Lepista sordida protoplast monokaryons and a special primer pair SR-1*3 therefor. The method comprises the following steps: respectively taking genome DNAs (deoxyribonucleic acid) of two Lepista sordida protoplast monokaryons to be identified as templates, performing PCR (polymerase chain reaction) amplification by using a PCR primer pair composed of two single-chain DNAs as shown in SEQ ID NO.1 and SEQ ID NO.2, and detecting the sizes of the obtained PCR products; if both or neither of the PCR products of the two Lepista sordida protoplast monokaryons to be identified contains a DNA segment of 500-800bp, indicating that the mating types of the two Lepista sordida protoplast monokaryons to be identified are the same; and if one of the two contains a DNA segment of 500-800bp and the other does not contain a DNA segment of 500-800bp, indicating that the mating types of the two Lepista sordida protoplast monokaryons to be identified are different.
priorityDate 2013-05-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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