| abstract |
According to the invention, delta-12 desaturase gene (FADS12, d12) from mortierella alpine ATCC32222 (M. alpina ATCC32222) is amplified, such that multi-copy expression plasmid with inserted opai/d12 co-expression gene and recombinant Yarrowia lipolytica yeast strain are prepared. According to the invention, the recombinant Yarrowia lipolytica yeast strain assists in increasing endogenous substrate linoleic acid (LA) amount and outer soybean oil increased substrate LA amount in fermentation medium, through expressing d12. In the strain, opai gene and d12 gene expression and transcript levels are increased with a multi-copy integration manner, such that conjugated linoleic acid t10 and c12-CLA specific isomer synthesis amount in recombinant Yarrowia lipolytica yeast strain can be further improved. |