http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103215307-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6c6d22af4bf4e1b2205a929549da46d3 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
| filingDate | 2012-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b937229dc5e442ed1f140ce50d12163 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a257aca6b6673affca0e2bce5fd998d0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_636f2e8271bb8569780c5f46fc34c468 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3204651dd529c0619dac6dc11661bed2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_267f7ee5eefc108cc9d324e475809331 |
| publicationDate | 2014-07-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103215307-B |
| titleOfInvention | Genetic transformation method of agrobacterium-induced plum blossom mature cotyledon regeneration system |
| abstract | The invention belongs to the technical field of plant gene engineering and relates to a genetic transformation method of an agrobacterium-induced plum blossom mature cotyledon regeneration system. The genetic transformation method comprises genetic transformation, screening regeneration, GUS dyeing detection and PCR detection. The genetic transformation method is characterized in that through agrobacterium mediation, a GUS reporter gene and a PmCBFb gene are induced into acceptor plum blossom mature cotyledon cells; different factors influencing plum blossom mature cotyledon transformation are screened; the transformed acceptor cells are screened by kanamycin as a selector; and through GUS dyeing or PCR detection as auxiliary screening methods, a transgenic plum blossom plant is obtained. The genetic transformation system realizes direct culture of a transgenic plant without somatic embryo generation and obviously improves plum blossom genetic transformation efficiency. |
| priorityDate | 2012-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 63.