http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103215306-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e164bcc4bd54075650f89b687ea057ff |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 |
| filingDate | 2013-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-01-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b0489be85339a18e6b81a7a20c5c218f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e8bef7c26d1a0dfb66a818b14468e1b9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2d6055f0d6450197686429b09f514f8e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e57c3b19dca0326a0dc82dc6bb14dd9b |
| publicationDate | 2015-01-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103215306-B |
| titleOfInvention | Plant expression vector of serine acetyltransferase gene and application thereof |
| abstract | The invention discloses a special plant expression vector 1300-superpromoter-AgSAT which contains celery serine acetyltransferase gene and is capable of improving the high NO3<-> resisting capacity of a plant. According to the invention, the SAT gene is cloned from celery by an RACE method, over-expressed in tobacco leaf by a constitutive super-promoter, and transferred to wild tobacco by a leaf disc transformation method. Experimental results show that the SAT gene in transgenetic tobacco can be normally transcribed, and in a high NO3<-> environment condition, the growing condition of the transcribed SAT gene is better than that of an adjoining tree (non-transgenetic wild type), so that over-expression of the SAT gene can improve the enzymatic activity of SAT and GSH (Glutathione) synthesizing quantity in transgenetic tobacco to a great extent, and meanwhile, the activity of reductase of glutathione is enhanced to further lead to increase of GHS content. Therefore, the oxidation resistance of transgenetic tobacco is more effectively improved, so that the tolerance to high nitrate is enhanced. |
| priorityDate | 2013-04-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 102.