http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103215295-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_70fbe08b7c540157200696115221f654 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5f169b1cbb3b65b81e55fb85405af2e8 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 |
| filingDate | 2013-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_81079fde246b9d63e04917a3e58f1968 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9de97eb194c6f24d3f0d5f2c9d2d534e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_95de797eada15572769df1865bc87ccf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e60c80cf08f268ff51c10e9d0b2b3d16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7f3966633185de05349eb90a36af6ec5 |
| publicationDate | 2015-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103215295-B |
| titleOfInvention | Targeting vector for integrating Lys gene at fixed point of bate-casein locus and cells constructed thereby |
| abstract | The invention discloses a targeting vector for integrating a Lys gene at a fixed point of a bate-casein locus and cells constructed thereby. The targeting vector comprises a target gene Lys, wherein a 5' homologous arm and a 3' homologous arm are respectively arranged upstream and downstream of the target gene Lys, and homologous sequences at 700-850bp are respectively extended upstream and downstream of zinc nuclease recognition site of a second intron of cow beta-casein locus as the 5' homologous arm and 3' homologous arm. The targeting vector pCSN2-Lys-Neo-EGFP and a zinc nuclease expression vector are co-transfected to cow fetus fibroblast by an electric perforating method. Targeted positive clone cells are obtained through G418 medicine screening and PCR (Polymerase Chain Reaction) analysis, and the targeted positive clone cells as a core donor which is transferred to a cow denucleated oocyte to obtain a transgenetic clone embryo. Therefore, the invention lays a solid foundation for researching and developing anti-mastitis transgenetic dairy cow. |
| priorityDate | 2013-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 338.