http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103215235-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5655b1d2b3894fa96dc42b2fed95a8e0 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-52 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-805 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
| filingDate | 2012-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4aa46cca00ededb7a9b4fe128700b4dd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d91f3fe364db51e7cd5b287496d06b46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_28ad19bf91d79d6a98f5e4d39bb37314 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_781864512e853d369a5b904b9efa637b |
| publicationDate | 2014-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103215235-B |
| titleOfInvention | 3-imidazolyl tyrosine translation system and use thereof |
| abstract | The invention relates to an aminoacyl-tRNA synthetase mutant. The aminoacyl-tRNA synthetase mutant has an amino acid sequence composed of amino acids shown in the formula of SEQ ID NO.2 and their conservative mutants. The invention provides a 3-imidazolyl tyrosine translation system for specific and fixed-point insertion of 3-imidazolyl tyrosine (imiTyr) into a target protein by utilization of an orthogonal tRNA, an orthogonal aminoacyl-tRNA synthetase and their complex, and also provides a method for specific and fixed-point insertion of the 3-imidazolyl tyrosine into the target protein by the 3-imidazolyl tyrosine translation system. The 3-imidazolyl tyrosine translation system comprises 1), 3-imidazolyl tyrosine, 2), the orthogonal aminoacyl-tRNA synthetase, 3), the orthogonal tRNA, wherein the orthogonal aminoacyl-tRNA synthetase is obtained by preferential aminoacylation of the orthogonal tRNA by the 3-imidazolyl tyrosine, and 4), a nucleic acid for encoding a target protein, wherein the nucleic acid contains at least one selection codons specifically recognized by the orthogonal tRNA. The invention also relates to a method for specific and fixed-point insertion of 3-imidazolyl tyrosine in Myoglobin (Mb) so that the Myoglobin has oxidase activity, and a use of the method. |
| priorityDate | 2012-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 723.