http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103214562-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8cf3596a85bf4b78b77c3003ba09a0a7 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-445 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-085 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P1-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P1-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P35-00 |
| filingDate | 2008-09-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9ef80026f8acb314877eb07d61d51440 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0c054560457fd2790921c59c6c4c5c2e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fb1d49e07072c347db71cd5c07b8772e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_67f339331405cad6835dc89c588e8422 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5593e26ea2c3bc0962f3bef52f5c6be6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_00f6286b0b1ebb50d4483e5471931e4e |
| publicationDate | 2014-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103214562-B |
| titleOfInvention | Attenuated enterotoxin C2 superantigen mutant protein as well as preparation method and application thereof |
| abstract | The invention belongs to the field of the gene engineering, and concretely relates to a attenuated enterotoxin C2 superantigen mutant protein as well as a preparation method and application thereof. The attenuated enterotoxin C2 superantigen mutant protein has a base sequence in the sequence table SEQ ID No: 7, SEQ ID No: 11, SEQ ID No: 13 or SEQ ID No: 15. A staphylococcus aureus DNA is used as a template, and a primer pair is used for PCR amplification, and then a sec 2-F and a sec 2-R are used as primers, and then an obtained PCR product is used as a template of PCR amplification for obtaining a mutant gene, and the mutant gene is cloned into a pET-28a for constructing a genetically engineered bacterium of the attenuated enterotoxin C2 mutant protein. The mutant protein according to the invention is based on maintenance of a certain superantigen activity, and the emetic and pyrogenicity activities are substantially reduced than those of the wild type enterotoxin C2 protein, thereby achieving the purpose of toxic and side effect reduction. |
| priorityDate | 2008-09-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.