http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103212377-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bff16132c8da634f56320aa3f80c5089 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J20-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J13-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J20-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J20-28 |
| filingDate | 2013-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_26f72dc6c626b7e074e018f36c2693c9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_57da0e6286ccd9bd73a8635c6b48bea5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5f1f9ebab61fd073c156d9f232763fd0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_072e96a5936b1815d4a30b62d7a0dc29 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d96e1842e80e220246a5ef525dc2aa62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_74486bb0c92de28087c9607d0180a66d |
| publicationDate | 2014-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103212377-B |
| titleOfInvention | Preparation method of agarose immune magnetic microspheres and applications thereof |
| abstract | The invention discloses a preparation method of agarose immune magnetic microspheres and applications thereof, and relates to a preparation method of microspheres and applications thereof, which solves the technical problems that an existing purified monoclonal antibody is complex in process, is low in yield, and is high in cost, and coupling agents are hypertoxic. The preparation method comprises the following steps of: 1. preparing a ferric oleate precurser; 2. preparing a magnetic fluid; 3. preparing an oil phase and a water phase; 4. preparing agarose magnetic microspheres; 5. preparing activated and crosslinked agarose magnetic microspheres; 6. preparing agarose magnetic microspheres joined with spacer arms; and 7. preparing agarose immune magnetic microspheres. The agarose immune magnetic microspheres provided by the invention are used for purifying genetic engineering recombination trastuzumab monoclonal antibodies. The agarose immune magnetic microspheres prepared by the invention are used for the field of separating and purifying the monoclonal antibodies. |
| priorityDate | 2013-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 74.