http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103207167-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_eda37409f26cb8e833ba67f2b1bc1fd5 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2012-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_abd1932a9db03af8d7815d80196f5f28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a6eb43d4bfae01c9ab1249f6c74ea57f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fd11079a226bf73c0741040285ff3747 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9c0c9d0f0f2cc4fb95e47b481fbe735d |
| publicationDate | 2015-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103207167-B |
| titleOfInvention | Preparation method of fluorescence resonance system for rapid detection of ATP in mitochondria |
| abstract | The invention provides a preparation method of a fluorescence resonance system for rapid detection of ATP in mitochondria. The method comprises the following steps: step 1, providing a Cy3 fluorescence-labeled ATP-aptamer and an MTS sequence; step 2, performing a coupling reaction for QDs with a complementary strand of the ATP-aptamer and the MTS to obtain QDs molecular probes, wherein QDs is water-soluble quantum dots on carboxyl surface (COOH-QDs) and has a concentration of 1 [mu]M; and step 3, reacting the QDs molecular probes obtained in step 2 with the Cy3 fluorescence-labeled ATP-aptamer to obtain the fluorescence resonance system after interaction of the ATP aptamer and the complementary strand. According to the invention, the method utilizes characteristics of specific binding of the ATP and the aptamer thereof as well as a resonance energy transfer technology, and thus realizes rapid detection of the ATP and also realizes real-time monitoring for changes of ATP concentration in mitochondrial through specific transmembrane peptides-mitochondrial targeting fusion sequence (MTS), thereby providing a novel method for detection and monitoring of the ATP in vitro and in vivo. |
| priorityDate | 2012-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 17.