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filingDate 2013-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2014-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_97590d2208216f41a4eb8cd443091989
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publicationDate 2014-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-103205509-B
titleOfInvention High-flux non-diagnostic detection method for 13 respiratory viruses based on novel suspension chip technology
abstract The invention discloses a high-flux non-diagnostic detection method for 13 respiratory viruses based on a novel suspension chip technology. Aiming at the specificity gene sequences of 13 pieces or types of respiratory viruses, such as influenza viruses A and B, auxiliary influenza viruses 1 and 3, coronavirus SARS-CoV and CoV-NL63, respiratory syncytial viruses A and B, adenoviruses B and E, a rhinovirus, a human metapneumovirus and an enterovirus, 13 pairs of primers and corresponding specificity probes are designed; by multiple polymerase chain reaction (PCR) amplification and TSPE reaction, X-TAG nucleic acid fragment and biotin are respectively marked with corresponding gene fragments; the marked fragments are combined with corresponding encoded micro balls; and a suspension chip scanner is used for performing detection. The high-flux non-diagnostic detection method can accurately detect 13 respiratory viruses and subtypes of the 13 respiratory viruses; the probes do not generate cross reaction; the specificity is high; and the sensitivity is high. A high-flux, quick and accurate technical platform is capable of simultaneously detecting various respiratory viruses; technical equipment is supplied to the detection of pathogens of sudden respiratory tract infectious diseases; and information can be timely supplied to the handling of infectious disease epidemic situation burst.
priorityDate 2013-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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