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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02
filingDate 2011-12-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2014-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e429a3f73a8a9b3f89b0eb5c7f7ec2c7
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publicationDate 2014-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-103205477-B
titleOfInvention Method for producing recombinant duck-beta-defensin-2 protein
abstract A method for producing a recombinant duck-beta-defensin-2 protein. The method is characterized by comprising an in vitro inducible expression process of a yeast recombinant converter containing duck beta-defensin-2. The process is as below: (1) induced expression of a positive yeast converter. Results show that mycelium protein subjected to induced culture has a quite obvious expression band of 4.32kD compared with a control group, which is not induced; through efficient liquid chromatography-tandem mass spectrometry identification, the mycelium protein has an amino acid sequence consistent with that of an objective protein; therefore, the target gene has been expressed, and the recombinant duck beta--defensin-2 protein has been produced. Compared with the prior art, the invention has advantages of antibiosis, growth activity promoting, suitability for application in animal production, low cost and high production efficiency.
priorityDate 2011-12-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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