http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103204936-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_29563bf48d4d52290d08117d5ad5ed45 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-06 |
| filingDate | 2013-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_261e29b618e7c9deb455caab43a39dad http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_395cddbae26d8154d7eca8e8bfb41fef http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a6ef756acb9a43b0d551acb651c3567d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aa03d574fb72945804d4767d02742530 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_50a53375f1bdb24624dbe3cc230fa114 |
| publicationDate | 2014-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103204936-B |
| titleOfInvention | Preparation method for polyclonal antibody to salmonella effect protein SopB |
| abstract | The invention discloses a preparation method for a polyclonal antibody to salmonella effect protein SopB. The invention aims to provide the preparation method for the rabbit-derived polyclonal antibody with good specificity, high sensitivity and capability of specifically binding to the endogenous salmonella effect protein SopB secreted in the process of salmonella infection of a host cell. A protein sequence as represented by SEQ ID No. 2 is used as immunogen, wherein the protein sequence consists of 139 amino acids located between position 29 and position 168, counted from terminal N, of a full-length amino acid sequence as represented by SEQ ID No. 1 of the salmonella effect protein SopB, and a conventional preparation method for a polyclonal antibody is used for preparation of antiserum; then the antiserum is purified so as to obtain the polyclonal antibody to the salmonella effect protein SopB. According to the invention, the polyclonal antibody to the salmonella effect protein SopB is prepared by using the method, and the polyclonal antibody is beneficial for immunoblotting analysis and immunofluorescent staining laser confocal microscopic observation of the salmonella effect protein SopB and for in-depth research on the infection mechanism of enteropathogens. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109313161-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109313161-A |
| priorityDate | 2013-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 73.