http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103202229-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_37e7d7638243c165d37f8ad592ebbc0c |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 |
| filingDate | 2013-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b4654060d1da548ba57c4b0f1dae8489 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2d8afb12384a457bb5b37eaa76577013 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bb0a349c5649034b5dc226712f01209b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_07f54e6afb30b289aeba49b52d51f6d2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4474e760550713744ebcdc069846d0b3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e2ebc24f5d234276511cc9bcd43f33cf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0ea86415d408d1e6e33fa721d4879571 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5e322968055d111de29a262eeefb0f25 |
| publicationDate | 2014-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103202229-B |
| titleOfInvention | Tissue culturing and rapid propagating method for chloranthy florida var. plena |
| abstract | The invention provides a tissue culturing and rapid propagating method for chloranthy florida var. plena. The tissue culturing and rapid propagating method comprises the following steps of: (1) washing stem tips of caulicles of chloranthy florida var. plena with sterile water on an ultra-clean table, immersing the washed stem tips by 75% of alcohol for 20-30 seconds, immersing and disinfecting the stem tips by 0.1%-0.2% of mercury bichloride for 6-10 minutes, and inoculating the stem tips, which are washed by the sterile water, into a cluster bud induction medium for primary induction culturing; (2) dividing cluster buds obtained through primary induction culture into small individual buds, and inoculating the small individual buds into a cluster bud propagation culture medium for subculturing; and (3) inoculating bud seedlings into a strong seedling rooting culture medium for rooting culturing when the bud seedlings grow to 2cm-3cm. The method provided by the invention can be used for rapidly propagating chloranthy florida var. plena, not only can save costs for culturing seedlings and improve production efficiency, but also is not restricted by external conditions, can be carried out in four seasons, saves seedling occupied area, can form a great amount of excellent test-tube seedlings in a short time, and can be used for large-scale and industrialized production. |
| priorityDate | 2013-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 35.