http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103196982-B
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_94b3e3452cea93da522d25e041f55619 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-447 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-34 |
filingDate | 2013-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2014-08-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9d2807b529db0446286df44b0de41856 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c40702b3fc2daaecf19cb93cc6fba052 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1614329011eb0642176d018a7ce3b3e2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f1253c743adcb83d3bbea6344fbdefb4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1c04411070604a7b2e59f9e91a897991 |
publicationDate | 2014-08-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103196982-B |
titleOfInvention | Method for determining content of pyrroloquinoline quinone in biological sample by magnetic solid-phase extraction-capillary zone electrophoresis |
abstract | The invention provides a method for determining the content of pyrroloquinoline quinone in a biological sample by magnetic solid-phase extraction-capillary zone electrophoresis and belongs to the field of a life analyzing technology. According to the method disclosed by the invention, SiO2 and adsorbents with different polarities are mixed; and the adsorbents have different selective properties on compounds with different hydrophobic properties, so that the effective separation can be carried out. A capillary electrophoresis method adopted by the invention adds a modifying agent beta-cyclodextrin; trifluoroacetic acid is firstly added into a capillary electrophoresis buffering system to solve the trailing problem of a peak in a capillary electrophoresis picture; and the symmetrical property of the peak is obviously better than that of a document report. The method disclosed by the invention is rapid and convenient, and can be used for determining the content of the PQQ in milk by utilizing a magnetic solid-phase extraction technology being combined with capillary electrophoresis firstly. Compared with a document method, the method disclosed by the invention improves the separation efficiency by 6 times. |
priorityDate | 2013-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 67.