http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103194507-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cb351a5a4e03ee3773bd0df5dbae9ac3 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-14 |
| filingDate | 2013-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8d0d6e6ced6bd124385b7a2758d8529e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_afbbbe1464c948e9f8d7b93e010180cd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_57619dafbd43b56d5efb251c4df81d0a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cb7bd61cf7aecd1a76053331c5d442e8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e8c923445d73f73456a1aa7d0ccc5b87 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a0644b4eadad52270068b158708a7af6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_743ec8a912a077873726ddb060d9d56b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f3b7ed0c8e5bd8d43b5612694ae22224 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dd04949b7a3a1a414461229ea6d83802 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fe74924b030d653dc644e554f043c89b |
| publicationDate | 2014-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103194507-B |
| titleOfInvention | Method for continuously preparing beta-cyclodextrin by adopting manila immobilized cells |
| abstract | The invention discloses a method for continuously preparing beta-cyclodextrin by adopting manila immobilized cells, belonging to the technical field of production of beta-cyclodextrin. The method disclosed by the invention comprises the following steps of: weaving manilas into a manila curtain and subsequently treating the manila curtain, fixing cyclodextrin glycosyl transferase CGTase producing bacterium ATCC21783 on the manila curtain, shaking a flask and producing CGTase, taking the manila curtain out every 2-3 days, washing the manila curtain by using normal saline to remove aged and degraded colonies from the manila curtain and putting the manila curtain into a fresh culture medium for continuous culture; using the CGTase produced by fermenting in production of the beta-cyclodextrin according to industrial steps. The continuous production of CGTase is facilitated by the manila immobilized cells; and further the continuous production of beta-cyclodextrin is realized. The process can last for more than one month. According to the method disclosed by the invention, the manila serving as a material which is natural and easily available is used as an immobilized substrate for the first time and used for immobilized CGTase producing bacteria; and the method disclosed by the invention is simple in immobilizing process and prominent in continuous production effect. Compared with the existed preparation process of beta-cyclodextrin, the method disclosed by the invention has the advantages of shortening the enzyme production period of the strain, simplifying the preparation process of the beta-cyclodextrin and improving the production efficiency. |
| priorityDate | 2013-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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