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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C403-24
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filingDate 2013-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2015-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dc837c6ebd0c1d4c7a3c31ab9663d9a8
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publicationDate 2015-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-103194459-B
titleOfInvention Transgenic chlorella with high biomass and/or high lutein yield and preparation method thereof
abstract The invention discloses a transgenic chlorella with high biomass and/or high lutein yield and a preparation method thereof. The invention provides a method for culturing the transgenic chlorella. The method is carried out by importing a vitreoscilla hemoglobin coding gene to a target chlorella to acquire the transgenic chlorella, wherein the biomass and/or lutein yield of the transgenic chlorella are/is greater than that of the target chlorella; and the amino acid sequence of the vitreoscilla hemoglobin is a sequence 2 in a sequence table. The experiment of the invention proves that a vitreoscilla hemoglobin coding gene expression cassette is imported to a wild chlorella through the method disclosed by the invention so that the transgenic chlorella is acquired; compared with the biomass of the wild chlorella, the biomass of the transgenic chlorella disclosed by the invention is observably improved by 38.81% in comparison with the lutein content of the wild chlorella; the lutein content of the transgenic chlorella is slightly improved by 5.93% in comparison with the lutein content of the wild chlorella, and the lutein yield of the transgenic chlorella is greatly improved by 47.19% in comparison with the lutein yield of the wild chlorella, so that the transgenic chlorella is a chlorella with high biomass and/or high lutein yield.
priorityDate 2013-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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