http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103194442-B
Outgoing Links
Predicate | Object |
---|---|
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A40-70 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06 |
filingDate | 2013-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-02-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-02-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103194442-B |
titleOfInvention | Novel polypeptide, hydrolysis antibacterial peptide and preparation method thereof |
abstract | The present invention relates to the preparation method of a kind of novel polypeptide and restructuring hydrolyzed peptide thereof, be specially antibacterial peptide and preparation method thereof, is its aminoacid sequence as SEQ? ID? shown in NO:2; The present invention is according to pepsic hydrolysis properties, engineer one can by the polypeptide of its good hydrolytic, according to the codon preference of e. coli strain bl21, chemical synthesis coding sequence, be cloned in pGEX-4T-1 plasmid, be transformed into BL21 bacterial strain, with IPTG induction, realize with the glutathione-S-transferase of occlusion body form high expression (GST) fusion rotein.Collect abduction delivering thalline, under pepsin hydrolysis effect, discharge the pepsic activated antibacterial peptide of tolerance.Recombined new polypeptide and hydrolyzed peptide thereof can be used as additive for farm animal feed or for the prevention of livestock and poultry and treatment. |
priorityDate | 2013-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 34.