http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103184290-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_550246a1a90f3c3034cf90d3392188d9 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2013-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c11ff3dc54f6cffb50803ba7a7e0b4a7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e3fd87f3d24c75ccc88e9cd438b0bcda http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4d9856d19df7586a12e53bd73cbcea30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d94d5c13f091b20f6faaed5a9dafe6f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6397e7aa35e4e178b6623ce7fdf1a0f1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d8725f0388a3677ddfec02c75eb1b00c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1f73a6a89a635de306430e2295d77577 |
| publicationDate | 2014-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103184290-B |
| titleOfInvention | Marking method of applying SSAP molecular marker technology to peony |
| abstract | A marking method of applying an SSAP molecular marker technology to a peony comprises the following steps: designing primers, extracting peony genome DNA, performing enzyme digesting for peony genome DNA, preparing a connector, connecting enzyme digested product with the connector, pre-amplifying of connected product, conducting a selective amplified reaction, testing polyacrylamide gel electrophoresis and the like. According to the marking method, pony genus retrotransposon LTR sequence primer is designed, and through the test of the polyacrylamide gel electrophoresis, stripes are rich; and compared with LTR primer of other species, the primer can directly used to polymorphism analysis between pony genuses, so as to achieve high efficiency and practicability; the selective amplified system is optimized and more suitable for the amplification of pony genus SSAP molecular marker; and silver staining method of polyacrylamide gel electrophoresis is improved, and fixing and dying steps of silver staining are combined to further improve the efficiency of applying the SSAP molecular marker on the pony genus. |
| priorityDate | 2013-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 73.