http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103184262-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d40285e27f4ed83b8728b0d477404958 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 |
| filingDate | 2013-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-01-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e4254d8215306573ac47bbcfba0bb0bd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c88fa45d1d23b33ff1b345bfb24a4fed http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b86785746036cc02157115f6b14fe8ad http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ff9c7464c0e9043fbf5d91b0a438cfd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b26f262fc08666977b590123d7502df9 |
| publicationDate | 2015-01-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103184262-B |
| titleOfInvention | Method for extracting and purifying pepsin-soluble collagens of black sea cucumber in East China Sea |
| abstract | The invention provides a method for extracting and purifying pepsin-soluble collagens of black sea cucumbers in East China Sea. The method comprises the following steps: soaking a clean, massive black sea cucumber sample in East China Sea with an EDTA (Ethylene Diamine Tetraacetic Acid) solution; performing homogenized homogeneity; performing extraction in sequence by using a Tris-HCl solution, a NaOH solution and a pepsase acetic acid solution; and performing salting out and dialysis to obtain the pepsin-soluble collagens. According to the invention, the EDTA chelating agent is used to remove chemical element with high pollution concentration from sea cucumber tissues, and the removal efficiency can reach more than 90%, a crosslinking effect in chemical bonds and among the chemical bonds of a collagen albumen product can be effectively removed after pepsase is used for performing hydrolysis on collagen fibers, so that maximally extracting the solvation collagen albumen can be realized; after the purification steps of sodium chloride salting out and dialysis, and the like are carried out, and the purity of the obtained collagen albumen product by extraction can reach more than 90%, the yield can reach 56.99-61.52% (dry weight), which is 97.1-102.5% of the total collagen albumen content of the black sea cucumber in East China Sea, so that the extraction of collagen albumen content with high productivity of the black sea cucumber in East China Sea can be realized. |
| priorityDate | 2013-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 70.