http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103183720-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 |
filingDate | 2012-08-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2015-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2015-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103183720-B |
titleOfInvention | The extracting method of low molecular peptide in a kind of milk and milk products |
abstract | The present invention relates to the extracting method of low molecular peptide in a kind of milk and milk products.The method that object is to provide has the advantage that extraction effect is good, purifying property is good.Technical scheme is: carry 1): with damping fluid by fresh milk or milk powder dilute 1 ~ 5 times, removes lipid and breast and the macromolecule protein in milk-product and other particulate matter, obtains low molecular peptide just extracting solution; 2) just purify: by first extracting solution through extraction and decon; Then eluted by the low molecular peptide on column extractor, collect elute soln, last rotary evaporation falls methyl alcohol in elute soln or acetonitrile, obtains low molecular peptide just scavenging solution; 3) purify again: the first scavenging solution pH value obtained is transferred within 2 ~ 4, then low molecular peptide adsorbed and be enriched on this solid-phase resin, subsequently uncharged small molecule organic compound and impurity are washed away, then use ammoniacal liquor methanol solution wash-out solid-phase resin, collect elutriant.4) evaporate: obtain highly purified low molecular peptide mixture. |
priorityDate | 2012-08-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 38.