http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103175818-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d6068f2f9b7c230583fb05b301f10881 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 |
| filingDate | 2013-03-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_586c3947a022e61f9097b8ff07ae5ef1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bc34f7e4d0e3d4614047866e8aab583f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_28d6b990ff66f3c58c511f309a2ca5fc |
| publicationDate | 2014-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103175818-B |
| titleOfInvention | A method for measuring melamine |
| abstract | The invention discloses a method for determining melamine. Add 0.05-5.0mL 0.1-0.5g/L melamine solution, 1.0-2.5mL 4.0× 10-6 mol/L eosin Y solution, 0.5-1.0mL 2.0×10 -4 mol/L neutral red solution and 0.3-1.0 mL pH3.5 Beritan-Robison buffer solution, using the solution without melamine as the reagent blank, dilute to the mark with ultrapure water, and store at 30°C After reacting in a water bath for 30 minutes, measure the fluorescence value F 538nm of the solution containing melamine and the fluorescence value F 0 of the reagent blank at 538nm on a fluorophotometer with a 1cm quartz cuvette and use 520nm as the excitation wavelength, and calculate the difference ΔF 538nm = F 538nm - F 0 value. Take 30mL of milk, add 20.0mL of 1% trichloroacetic acid solution and 8.0mL of 2% lead acetate solution, sonicate for 20min; take part of the solution and centrifuge for 10min, take 1mL of supernatant, measure the fluorescence value according to the above method, and calculate the content of melamine in milk . The method of the invention is simple and quick. |
| priorityDate | 2013-03-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 27.