http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103173512-B
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6dd935636c59998dbebd32cb9c3b3300 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_236abd6acedb34e076423324569e9b50 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 |
filingDate | 2013-03-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2015-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_936919990c4c2d61fadda2d37befa0f7 |
publicationDate | 2015-04-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103173512-B |
titleOfInvention | Process for extracting Penis et Testis Canis polypeptide through enzymatic method, and obtained Penis et Testis Canis polypeptide extract |
abstract | The invention provides a process for extracting a Penis et Testis Canis polypeptide through an enzymatic method, and an obtained Penis et Testis Canis polypeptide extract. The process comprises the following steps: 1, enzymatic extraction of an animal medicine material Penis et Testis Canis: carrying out dipping extraction of rubbed Penis et Testis Canis through using a protease preparation added aqueous solution as an enzymatic extraction liquid at 30-55DEG C for 0.5-6h; and 2, alcohol extraction: adding ethanol to the Penis et Testis Canis solution obtained after the above enzymatic hydrolysis to prepare an ethanol extract liquid having a volume concentration of 30-60%, carrying out heating reflux at 50-90DEG C for 1-5h, carrying out reflux extraction of residues with the ethanol liquid having a volume concentration of 30-60% for 1-4h, and mixing the above obtained extract liquids. The Penis et Testis Canis polypeptide extract obtained through using the process has complete active components; and compared with simple ethanol heating reflux, the process allows the extraction to be improved by above 40%, and has the advantages of mild extraction conditions, simple operation and low cost. |
priorityDate | 2013-03-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.