http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103173483-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-72 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 |
| filingDate | 2012-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103173483-B |
| titleOfInvention | Using Candida glycerolgenesis feature 5.8S sequences as the pURGAP vector constructions of integration site and its application |
| abstract | Structure and its application the invention discloses a kind of Candida glycerolgenesis integration vector pURGAP that can be cloned with homologous recombination.Constructed pURGAP carriers include yeast Candida glycerinogenes by structure with 5.8S rDNA sequence SEQ NO.2, yeast Candida glycerinogenes feature 5.8S sequence SEQ NO.1 sites can be integrated in, constructed pURGAP carriers also include osmotic pressure promoter P CgGAP , zeocin resistance markers and Escherichia coli height copy all elements.Compared with other integration vectors, pURGAP is remarkably improved integration vector transformation efficiency, and exogenous protein expression intensity is remarkably reinforced using feature 5.8S rDNA sequences as integration site.Foreign gene or promoter effectively can be carried out integrant expression by constructed pURGAP in Candida glycerolgenesis, are the integration efficiencies for improving expression vector, accelerated a kind of important method of industrial strain Candida glycerolgenesis modified recombinant speed. |
| priorityDate | 2012-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 22.