http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103160496-B
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7d13b253bd8988165c65d816362f7316 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_62fd70cba2aa0f3d0671e6f392868dd6 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate | 2013-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2014-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5c4b6c3ac6980a1254662dd69f0eda9f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_59120f42af5e402ae45247053be2924e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_37e94a07e9403a922c98b01798cd0774 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ca3265620b12b0311b924c15e5f57fef http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3475bd720c6a90200c785d0d23c13dd9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_348935cd6f9226f01f370f8c7f170182 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_71604a4c5e003aaee2d979aad9dd6864 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_52953c13fcf4a4867a8e674c911e2649 |
publicationDate | 2014-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103160496-B |
titleOfInvention | Method for extracting and purifying total RNA (ribonucleic acid) of blood of large-scale experimental animal |
abstract | The invention relates to a method for extracting and purifying the total RNA (ribonucleic acid) of the blood of a large-scale experimental animal. The method specifically comprises the following steps of: using the blood of the large-scale experimental animal as a sample; performing lysis on erythrocytes by using an improved erythrocyte lysing solution; and extracting the total RNA through a guanidinium isothiocyanate method, and purifying the total RNA by using an improved oligo (dT)-cellulose chromatography to finally obtain poly(A)+RNA of the blood of the large-scale experimental animal. The purified poly(A)+RNA obtained by the method has important and irreplaceable application values in the aspects of researching gene functions and gene regulation and control, clarifying pathogenesis of diseases, searching medication target spots and the like. |
priorityDate | 2013-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 51.