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filingDate 2013-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2014-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2014-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-103160496-B
titleOfInvention Method for extracting and purifying total RNA (ribonucleic acid) of blood of large-scale experimental animal
abstract The invention relates to a method for extracting and purifying the total RNA (ribonucleic acid) of the blood of a large-scale experimental animal. The method specifically comprises the following steps of: using the blood of the large-scale experimental animal as a sample; performing lysis on erythrocytes by using an improved erythrocyte lysing solution; and extracting the total RNA through a guanidinium isothiocyanate method, and purifying the total RNA by using an improved oligo (dT)-cellulose chromatography to finally obtain poly(A)+RNA of the blood of the large-scale experimental animal. The purified poly(A)+RNA obtained by the method has important and irreplaceable application values in the aspects of researching gene functions and gene regulation and control, clarifying pathogenesis of diseases, searching medication target spots and the like.
priorityDate 2013-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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