http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103146757-B
Outgoing Links
Predicate | Object |
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classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-87 |
filingDate | 2007-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2016-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2016-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103146757-B |
titleOfInvention | Mediation by polyethylene glycol will cause the improved method of mutagenesis of mutagenesis core base introduced plant protoplast |
abstract | The present invention relates to will cause the improved method of mutagenesis of mutagenesis core base introduced plant protoplast by the mediation of polyethylene glycol. particularly, the present invention relates to the method for the double-stranded receptor dna sequence of directed change in plant protoplast, it comprises that double-stranded receptor dna sequence and donor are caused to mutagenesis core base is combined, its double center chain receptor dna sequence contains the first DNA sequence dna and the second DNA sequence dna with the first DNA sequence dna complementation, wherein donor causes mutagenesis core base with respect to the double-stranded receptor dna sequence that will change, preferably comprise at least one mispairing with respect to the first DNA sequence dna, wherein the method further comprises that the conversion of use polyethylene glycol (PEG) mediation causes mutagenesis core base by donor and introduces the step in cell protoplast and use PEG protoplast transformation to increase directed mutagenesis rate. |
priorityDate | 2007-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 96.