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filingDate 2013-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2014-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8ee88e44e89213221b09b04dc0cf9345
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publicationDate 2014-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-103146741-B
titleOfInvention Three-stage genetic transcription control method for improving cellulosic ethanol yield and genetic engineering bacterial strain
abstract The invention relates to the field of biology preparation of ethanol, in particular to a three-stage genetic transcription control method for improving cellulosic ethanol yield and genetic engineering bacterial strain. The method comprises a step of expressing genes of XR, XDH, XK, RPE1, RKI1 and TAL1 in saccharomyces cerevisiae by building of expression plasmids of the genes of XR, XDH, XK, RPE1, RKI1 AND TAL1, wherein KGD1 is used for starting sub-mediation rate-limiting gene XK, and HSP26 is used for starting sub-mediation key rate-limiting gene TAL1. The cellulose alcoholic fermentation process is divided into three stages including an anaerobic glucose ferment stage, an aerobic xylose respiratory metabolism stage and a ferment later stage period which has a heat shock characteristic and gives priority to high temperature restraint. A target gene is enabled to be high efficiently expressed in each stage so that cellulose zymolyte can be high efficiently transferred to cellulosic ethanol.
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