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publicationDate 2014-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-103146720-B
titleOfInvention Trans-4-hydroxy-L-proline hydroxylase modifying gene with high transformation rate and application thereof
abstract The invention discloses a trans-4-hydroxy-L-proline hydroxylase modifying gene with high transformation rate and application thereof. An expression carrier of an optimization gene is constructed by optimizing nucleotide sequence of trans-4-hydroxy-L-proline hydroxylase (GenBankID:D78338.1) in Dactylosporangiumsp.RH1; the thallus is collected as the enzyme source of the transformation reaction through the prokaryotic expression. The experiment result expresses that the transformation rate of generating the trans-4-hydroxy-L-proline by using L-proline as a substrate is as high as 91%. The gene can be applied to biologically generating trans-4-hydroxy-L-hydroxyproline and has good industrial application value.
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