Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9a110c5a10bf456ca032940971d2a73c http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_27396494dbf2775fd87cdd0287df497f |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-24 |
filingDate |
2013-03-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2014-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f36f9011630c6c29f0235c84b929a52a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_549d2b08cfc4accfaff9f936241ddd6d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bf3eeca16f7941dc6a8a9ab35f82be9b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_571ccdbb9e6d423022db0909590115ae http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ad0281b7b4c598d47895d0907237736d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9455134c2a0b433012a4da3fab97d5f8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_29e990998c2dfc875889efabeb9d2d19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7fe741d060f6dbf9f29914b99e7ae947 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2bedb62eb538d939d7f3e9a447cdf388 |
publicationDate |
2014-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CN-103146720-B |
titleOfInvention |
Trans-4-hydroxy-L-proline hydroxylase modifying gene with high transformation rate and application thereof |
abstract |
The invention discloses a trans-4-hydroxy-L-proline hydroxylase modifying gene with high transformation rate and application thereof. An expression carrier of an optimization gene is constructed by optimizing nucleotide sequence of trans-4-hydroxy-L-proline hydroxylase (GenBankID:D78338.1) in Dactylosporangiumsp.RH1; the thallus is collected as the enzyme source of the transformation reaction through the prokaryotic expression. The experiment result expresses that the transformation rate of generating the trans-4-hydroxy-L-proline by using L-proline as a substrate is as high as 91%. The gene can be applied to biologically generating trans-4-hydroxy-L-hydroxyproline and has good industrial application value. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108102964-A |
priorityDate |
2013-03-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |