http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103141472-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c0855175d5678ed44ac8a25a3903fac8 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A40-81 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K61-00 |
| filingDate | 2013-03-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_93e1798496aede01ba43774ce4da8746 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_47a6a8355b85d9ddacd5393b1e78af3c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1cd02ca7cc1aaa9069c86a78693259c1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_86b753569eca89bcdcc10f531f898737 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1925a8bee1a69262c307f8180b29cd18 |
| publicationDate | 2014-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103141472-B |
| titleOfInvention | Ultra-low-temperature freezing preservation and activation method for Hong Kong oyster sperm |
| abstract | The invention discloses an ultra-low-temperature freezing preservation method for Hong Kong oyster sperm. The method comprises the following steps of collection, dilution and refrigeration of Hong Kong oyster sperm, and is characterized in that for the step of dilution, a diluent comprising a base liquid and an antifreeze agent is used for diluting and balancing the Hong Kong oyster sperm, for the refrigeration step, a programmed freezing manner is adopted for freezing, and finally the oyster sperm is preserved in liquid nitrogen. An activation method for the Hong Kong oyster sperm frozen and preserved by the method comprises the steps of defrosting and activating, wherein for the defrosting step, the sperm preserved in the liquid nitrogen is taken out and is completely defrosted in constant water being 35-55 DEG C, for the activating step, ammonia sea water with final mass concentration being 0.1563-0.6250%is added into recovered sperm. Due to the method, the rate of survival, vitality, fertility rate, and hatchability of the Hong Kong oyster sperm are improved, so that the breeding course of the Hong Kong oyster is accelerated, and the method is beneficial to the breeding of the Hong Kong oyster and large-scale breeding of fine breed, and has significance on preservation of Hong Kong oyster germplasm resources and conservation study of biodiversity. |
| priorityDate | 2013-03-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 47.