http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103114079-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a355bb82c581f2a4ecded4ee6f30e807 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-076 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 |
| filingDate | 2013-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1f1c50cf5b220bc9a4a7bf27fa0fae4f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ac3a2f07914c32fd1a128678c37de250 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d1c007801e505434df5262e9c24b65a9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_14a3e88dc549f77faeaa0ed953f32e44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_00734f567c5ecf6b022786f2f852a3ce |
| publicationDate | 2014-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103114079-B |
| titleOfInvention | Preparation of serologic H-Y antigen gene DBY antibody and XY sperm immune sorting method thereof |
| abstract | The invention discloses the preparation of a serologic H-Y antigen gene DBY antibody and an XY sperm immune sorting method thereof. The method comprises the following steps of: predicting and screening three B cell epitope antigen peptides of the H-Y antigen gene DBY antibody by a method in information biology, and connecting, recombining and performing prokaryotic expression to obtain high-purity DBY recombinant antigen peptide; immunizing a New Zealand white rabbit based on the recombinant antigen peptide as an antigen to obtain a polyclonal antibody high in valence and strong in specificity; and sorting about 63.2% of sperm, 4.5% plus or minus by DBY antibody fixation flow cytometry. According to the invention, the XY immune sorting method is established based on the serologic H-Y antigen gene DBY antibody, so that a simple, efficient and safe ideal sex selection method can be established by separating X and Y sperms by the antibody, therefore, genetic improvement of seed variety (or variety) is better controlled so as to obtain faster genetic progress and efficiency advantages on production and management in the animal husbandry. |
| priorityDate | 2013-01-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 27.