http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103114075-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0b37d6aef2848e1b99bc88d10e822f6a |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-073 |
| filingDate | 2013-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_77e5b23f60bcae16941cf1a1ebc287d1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_648430aff403ebf8cef8ebbdd1b6eede http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ffafebeb78a7adf41e5e27774e495e3f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_54997ff1220bba66444f43461c22abd5 |
| publicationDate | 2014-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103114075-B |
| titleOfInvention | Cattle trophoderm stem cell system establishment method |
| abstract | The invention provides a cattle trophoderm stem cell system establishment method which comprises the following steps: inoculating hemiblastula of which zona pellucida is removed through pronase treatment into a feed layer in mouse embryonic fibroblast subjected to mitomycin treatment and Wnt-3A mouse subcutaneous connective tissue cells, adding 2i small-molecule inhibitor culture solution to perform continuous cell culture of cells, and establishing a cattle trophoderm stem cell system. The method can be used for obtaining and culturing cattle trophoderm stem cells under in-vitro conditions for a long time. The cattle trophoderm stem cell system establishment method can be applied to large animal transgenosis and somatic cell cloning and can be further used for cattle embryo implantation and placenta differentiation. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-WO2016143866-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108251377-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108251377-B |
| priorityDate | 2013-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 69.