http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103103285-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3f10d19b1d8a3fe5441942f7cf32e057 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2013-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-02-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ed683a614b5f8a8dc8e782b3a48ab095 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0c37d1bfac01be44695dedeb4ad16718 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_469263d6b8b968632d3ed15ac7c73b9a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f6854845abd831229e9940f62cda3edb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_246542ac2a1a1dcaaa98eb7e965d1955 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e3dab0ea04aa10c469d4602f750d98a2 |
| publicationDate | 2014-02-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103103285-B |
| titleOfInvention | LAMP rapid detection method for Heterodera filipjevi and its application |
| abstract | The invention relates to an LAMP (loop-mediated isothermal amplification) rapid detection method for Heterodera filipjevi and its application. The method comprises: according to an RAPD specific fragment sequence of Heterodera filipjevi, designing and screening 6 LAMP primers, i.e. HF11-F3, HF11-B3, HF11-FIP, HF11-BIP, HF11-LB and HF11-LF, extracting the genome DNA of Heterodera filipjevi, establishing a loop-mediated isothermal amplification system and optimizing it, subjecting an amplification product to color development and observing it, thus rapidly detecting and identifying Heterodera filipjevi. The detection method provided in the invention has the advantages of strong specificity, high sensitivity, simple operation, high efficiency and fastness, as well as visually observable results, and has very high application value in rapid detection of Heterodera filipjevi and the early and field diagnosis aspect of Heterodera filipjevi disease. |
| priorityDate | 2013-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 70.