http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103103201-B

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filingDate 2013-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2014-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4f3618e1ef03fca83f4c4b8f03ddf7f2
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publicationDate 2014-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-103103201-B
titleOfInvention DNA (deoxyribonucleic acid) molecule, recombinant plasmid and escherichia coli
abstract The invention relates to the technical field of organisms, and discloses a DNA (deoxyribonucleic acid) molecule of a nucleotide sequence shown in SEQ ID NO:1, a recombinant plasmid pKR-E obtained by plasmid pKK223-3 and the DNA molecule inserted between the SmaI and HindI II enzyme cutting sites, and a recombinant plasmid pKTR-E obtained by plasmid pKK223-3-ThrABC and a segment composed of a Tac promoter, the DNA molecule, rrnB T1 Terminator and rrnB Terminator inserted between the BamHI and SphI enzyme cutting sites. The recombinant plasmid disclosed by the invention is converted into W3110 escherichia coli to obtain two strains with high L-threonine tolerance activity, and can be applied to fermentation of L-threonine.
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