Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_94f36b8d133d9521a9a2a8093a7aa321 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-08 |
filingDate |
2013-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2014-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4f3618e1ef03fca83f4c4b8f03ddf7f2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_776e4b991a70d5f9695cb766af55e5b9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_43961310347ced786f79452c32b44147 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_17846996333047046381142279d1e5d3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fea97bba59d07098d0a82e354ab6c8ff |
publicationDate |
2014-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CN-103103201-B |
titleOfInvention |
DNA (deoxyribonucleic acid) molecule, recombinant plasmid and escherichia coli |
abstract |
The invention relates to the technical field of organisms, and discloses a DNA (deoxyribonucleic acid) molecule of a nucleotide sequence shown in SEQ ID NO:1, a recombinant plasmid pKR-E obtained by plasmid pKK223-3 and the DNA molecule inserted between the SmaI and HindI II enzyme cutting sites, and a recombinant plasmid pKTR-E obtained by plasmid pKK223-3-ThrABC and a segment composed of a Tac promoter, the DNA molecule, rrnB T1 Terminator and rrnB Terminator inserted between the BamHI and SphI enzyme cutting sites. The recombinant plasmid disclosed by the invention is converted into W3110 escherichia coli to obtain two strains with high L-threonine tolerance activity, and can be applied to fermentation of L-threonine. |
priorityDate |
2013-01-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |