http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103103167-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_27396494dbf2775fd87cdd0287df497f |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2013-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9a2484edcc7156130a355436cdca9155 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_68f14b3cd7b92216916ace55fe87f799 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e6ea7f31eb2faf8f58abbf72de862baa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_83835ceac2253e2d6c19af76fb5b273c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1ee23c39a33877ead39ae7dd2c07dbd4 |
| publicationDate | 2015-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103103167-B |
| titleOfInvention | Mutant zymoprotein of D-amino acid oxidase and preparation method of mutant zymoprotein |
| abstract | The invention discloses a mutant zymoprotein of D-amino acid oxidase and preparation method of the mutant zymoprotein. The preparation method comprises the following steps: according to homologous sequence comparison result, respectively performing mutation on 115, 119 and 286 sites of D-amino acid oxidase in primary Arthrobacterprotophormiae through site-specific mutagenesis technology to construct three-site mutation expression vector Pet-e115A/N119D/T286A; and carrying out prokaryotic expression and purification to obtain zymoprotein. An apparent secondary speed constant Kcat/Km of the obtained mutant protein to substrate D-Met is 1.39*10 to the power of 5s-1.M-1, is 5.03 times of wild type DAAO (D amino acid oxidase), and is 55.96 times of pKDAAO protein of pig kidney source. The detection efficiency of partial D-amino acid can be improved, and the good application value is realized. |
| priorityDate | 2013-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 281.