http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103088434-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cb351a5a4e03ee3773bd0df5dbae9ac3 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-31 |
| filingDate | 2012-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b86e9924e2f011c34908ccc08815e688 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_213a46eb6a54b748ef0def7787e8a826 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_903c77a7a5f22250e6f9b130aaa2a014 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dc598e3a8e89569d06c745d805ff2cc7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_919a046b41883da1132b6b89e415dcca |
| publicationDate | 2014-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103088434-B |
| titleOfInvention | Construction method and application of Pichia stipitis large-fragment DNA (deoxyribonucleic acid) genome library |
| abstract | The invention provides a construction method of a Pichia stipitis large-fragment DNA (deoxyribonucleic acid) genome library, which comprises the following steps: (1) extracting genome DNA of Pichia stipitis of which the collection number is ATCC NO:58376; (2) constructing a recombinant vector; (3) screening a positive clone; and (4) performing enzyme cutting identification on the positive clone, and saving a library. The invention successfully constructs the Pichia stipitis large-fragment DNA genome library for the first time; the storage capacity of the library is high (the cloning probability is 97%), and the coverage rate is high (N=1.08); and a gene related to cellobiase can be successfully screened by using the genome library, thereby laying a foundation for the screening of an unknown functional target gene, the functional research on the related gene and the further reconstruction on the gene level so as to obtain an ideal industrially-produced strain. |
| priorityDate | 2012-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 58.