http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103088044-B
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0b37d6aef2848e1b99bc88d10e822f6a |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 |
filingDate | 2011-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2014-09-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_08b939b18689b20bb08a23f416a50acb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b41d81c042d3a905d06fa73a1e7ba3ae http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d17d26a38231c0b7c550f1d9442267e7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_54997ff1220bba66444f43461c22abd5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_77e5b23f60bcae16941cf1a1ebc287d1 |
publicationDate | 2014-09-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-103088044-B |
titleOfInvention | Targeting vector for knockout of bovine MSTN gene and application thereof |
abstract | The invention discloses a promoter trapping type targeting vector PIII-MSTN for knockout of a bovine myostatin MSTN gene. The vector can knock all of a first exon and a second exon and most of a third exon of the bovine MSTN gene; a knockout nucleotide sequence is shown as a SEQ ID NO:1; a PIII MSTN nucleotide sequence is shown as a SEQ ID NO:3, and contains homologous arm sequences about 1.3kb long and 6.8kb long of the bovine MSTN gene, a green fluorescent protein gene EGFP without promoter and a neomycin resistance gene Neor expression framework with promoter PGK. The promoter trapping type targeting vector PIII-MSTN provided by the invention can be used for the cultivation of transgenic beef cattle with MSTN gene knocked out. |
priorityDate | 2011-11-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 95.