| abstract |
The invention relates to the field of gene engineering, particularly a polygalacturonase PG8fn, and a gene and application thereof. The amino acid sequence is disclosed as SEQ ID NO.1 or 2. After the polygalacturonase PG8fn is treated at 35 DEG C and 45 DEG C for 60 minutes, the residual enzyme activity is still higher than 90%, which indicates that the enzyme has favorable heat stability. After Pichia pastoris expression, the specific activity is up to 28,122U/mg. |