http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103076452-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_17483d057b8e5a49f15b1130092bd694 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-60 |
| filingDate | 2012-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6396d80e4ce0dcada1967c355e47d5aa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2a7a5732c42de7a6385eb99c5ee8ed5a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_754be65393a0c83329a583b5801af764 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6de74c769af2165a82515437aa73c749 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_360c7c31ab84142cda6cf01e5ebe0bb7 |
| publicationDate | 2015-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103076452-B |
| titleOfInvention | Method for testing adiponectin and application thereof |
| abstract | The invention discloses a method for testing adiponectin. The method comprises the following steps: preparing a buffering liquid standard product APN, adding and dissolving 10 milliliters of blood plasma specimen into 4,990 milliliters and diluting until the ratio is 1:500 to obtain a sample to be tested; adding 100 milliliters of sample to be tested or the standard product into each tube, adding I-APN solution and rabbit anti-APN antibody, mixing completely, incubating at room temperature for 24 hours and passing the night; adding rabbit serum and goat anti-rabbit IgG antibody the next day, mixing uniformly, incubating at the temperature of 4 DEG C for 20 minutes, centrifuging for 20 minutes, and absorbing and discarding the supernate; measuring the a~ ray quantity of each test tube; drawing a standard curve, namely drawing the standard curve on logarithmic coordinate paper by taking the APN concentration of the standard product as horizontal coordinate and B/B0 as the vertical coordinate; finding the corresponding APN concentration from the sample B/B0 curve; and calculating the APN concentration. The test method is simple in steps and accurate in measurement. By the test method, the clinical significance of diabetes serum adiponectin for forecasting the macroangiopathy at the early stage of diabetes and the severity degree thereof can be evaluated by analyzing the influence factors of the macroangiopathy of different types of impaired glucose regulation people. |
| priorityDate | 2012-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 136.