http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103074431-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ab2d2e10c2da8c9230ed10dc86817a59 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2013-01-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_33e9c5d84d454e4636484ce384a8cc14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_53efca2dbc4f35af358f02d5636c6f6b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3d116b9a7492dc1fe0f62659241498e5 |
| publicationDate | 2013-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103074431-B |
| titleOfInvention | Special primer, kit and method for testing minRNA-128 in colorectal cancer serum |
| abstract | The invention discloses a special primer for testing the minRNA-128 in colorectal cancer serum. The special primer comprises a reverse transcription primer and a test primer of the miR-128, a reverse transcription primer and a test primer of U6 and a reverse transcription primer and a test primer of miRNA-16, as shown by SEQ IDNO: 1-9. A special kit for testing colorectal cancer serum minRNA-128 comprises the primers and a RNA separation solution. A method for testing the expression amount of the colorectal cancer serum minRNA-128 comprises steps: mixing a serum specimen to be tested with an isovolumetric RNA separation solution, centrifuging 16000g of the mixture for 10 minutes, and separating supernate; performing RT-PCR (reverse transcription-polymerase chain reaction); manufacturing a standard curve; and performing calculation to obtain a gene calibration initial copy number Q, and comparing the Q value of the miR-128 and the geometrical mean of Q values of reference gene U6 and miR-16 to obtain a relative expression amount of the minRNA-128. According to the method for testing the minRNA-128 in colorectal cancer serum, important references are provided for early discovery and early treatment of colorectal cancer. |
| priorityDate | 2013-01-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 69.