http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103074343-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-15 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-77 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2013-02-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-10-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2015-10-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-103074343-B |
| titleOfInvention | A kind of method utilizing the endogenous High-expression promoter of 2-DE technology screening Corynebacterium crenatum |
| abstract | Utilize a method for the endogenous High-expression promoter of 2-DE technology screening Corynebacterium crenatum, belong to proteomics and genetically engineered field.First the present invention identifies high expression level protein site in Corynebacterium crenatum born of the same parents by 2-DE combine with technique mass-spectrometric technique.Clone its encoding gene promoters, called after P-argC, P-argG, P-argF, P-ilvC and P-serA respectively, replace the tac promotor on shuttle vectors pDXW-8, and insert cat gene in its downstream, transformation of E. coli JM109 and Corynebacterium crenatum SYPA5-5 respectively, by measuring the activity of CAT, the activity of each promotor in recombinant bacterium: P-argG > P-argF > P-ilvC > P-serA > P-argC.This obtains from Corynebacterium crenatum composing type High-expression promoter first, for Corynebacterium crenatum high expression level foreign gene provides effective instrument. |
| priorityDate | 2013-02-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 71.